Abstract：In Britton-Robinson (BR) buffer solution at pH 4.0, erythrosine(ET) reacts with tetracaine hydrochloride (TA·HCl) to form an ion association complex that quenches the fluorescence of erythrosine and has a compound ratio of 1∶1. The measurement wavelength of fluorescence quenching was located at λex/λem=525 nm/556 nm. The linear range for TA·HCl is from 0.28 μg·mL-1 to 4.8 μg·mL-1,and its detection limit is 0.083 μg·mL-1. The influence of coexisting substance was also inspected, and the result shows that this method has a better selectivity. Therefore, a new fluorospectrophotometry, being high sensitive, sample and rapid, was developed to determinate TA·HCl at trace amounts. The method has been applied to the determination of TA·HCl in human serum and urine samples with satisfactory results. In this study, the spectral characteristics and the optimum reaction conditions were discussed with quantum chemistry AM1 method.
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